Review



313399 leaf purified anti mouse ly49h  (Bio X Cell)


Bioz Verified Symbol Bio X Cell is a verified supplier
Bioz Manufacturer Symbol Bio X Cell manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Bio X Cell 313399 leaf purified anti mouse ly49h
    (A) Histograms of <t>Ly49H</t> expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.
    313399 Leaf Purified Anti Mouse Ly49h, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/313399+leaf+purified+anti+mouse+ly49h/InVivoMAb+anti-mouse+TIM-1/pmc06614060-592-255-238
    Average 92 stars, based on 4 article reviews
    313399 leaf purified anti mouse ly49h - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Cytomegalovirus infection drives avidity selection of natural killer cells"

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells

    Journal: Immunity

    doi: 10.1016/j.immuni.2019.04.009

    (A) Histograms of Ly49H expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.
    Figure Legend Snippet: (A) Histograms of Ly49H expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.

    Techniques Used: Expressing, Infection

    (A-D) Experimental schematic (A). Equal numbers of splenic Ly49Hlo and Ly49Hhi NK cells, purified from congenically distinct WT mice, were co-transferred into Klra8−/− mice, and their responses tracked following MCMV infection of the recipients. (B) Flow plots gated on NK cells from blood at day 7 PI (left) and spleen at day 28 PI (middle). Quantification of percent Ly49Hlo and Ly49Hhi NK cells within total NK cells in blood at indicated days PI (right). (C) The percentage of Ly49Hlo and Ly49Hhi NK cells within transferred Ly49H+ NK cells in blood at indicated days PI (left) and in indicated organs at day 28 PI (right). (D) Histograms of Ly49H expression on Ly49Hlo and Ly49Hhi NK cells from blood at day 7 PI. Data are representative of two independent experiments with 3–5 mice per experiment.
    Figure Legend Snippet: (A-D) Experimental schematic (A). Equal numbers of splenic Ly49Hlo and Ly49Hhi NK cells, purified from congenically distinct WT mice, were co-transferred into Klra8−/− mice, and their responses tracked following MCMV infection of the recipients. (B) Flow plots gated on NK cells from blood at day 7 PI (left) and spleen at day 28 PI (middle). Quantification of percent Ly49Hlo and Ly49Hhi NK cells within total NK cells in blood at indicated days PI (right). (C) The percentage of Ly49Hlo and Ly49Hhi NK cells within transferred Ly49H+ NK cells in blood at indicated days PI (left) and in indicated organs at day 28 PI (right). (D) Histograms of Ly49H expression on Ly49Hlo and Ly49Hhi NK cells from blood at day 7 PI. Data are representative of two independent experiments with 3–5 mice per experiment.

    Techniques Used: Purification, Infection, Expressing

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Flow Cytometry, Purification, Recombinant, Staining, RNA Sequencing Assay, Expressing, Derivative Assay, Software, Imaging

    Related Articles

    Flow Cytometry:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Purification:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Expressing:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Infection:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Recombinant:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Staining:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    RNA Sequencing Assay:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Derivative Assay:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Software:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Imaging:

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAFTM Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAFTM Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.



    Similar Products

    92
    Bio X Cell 313399 leaf purified anti mouse ly49h
    (A) Histograms of <t>Ly49H</t> expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.
    313399 Leaf Purified Anti Mouse Ly49h, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/313399+leaf+purified+anti+mouse+ly49h/InVivoMAb+anti-mouse+TIM-1/pmc06614060-592-255-238
    Average 92 stars, based on 1 article reviews
    313399 leaf purified anti mouse ly49h - by Bioz Stars, 2026-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    (A) Histograms of Ly49H expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.

    Journal: Immunity

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells

    doi: 10.1016/j.immuni.2019.04.009

    Figure Lengend Snippet: (A) Histograms of Ly49H expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.

    Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAF™ Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAF™ Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Techniques: Expressing, Infection

    (A-D) Experimental schematic (A). Equal numbers of splenic Ly49Hlo and Ly49Hhi NK cells, purified from congenically distinct WT mice, were co-transferred into Klra8−/− mice, and their responses tracked following MCMV infection of the recipients. (B) Flow plots gated on NK cells from blood at day 7 PI (left) and spleen at day 28 PI (middle). Quantification of percent Ly49Hlo and Ly49Hhi NK cells within total NK cells in blood at indicated days PI (right). (C) The percentage of Ly49Hlo and Ly49Hhi NK cells within transferred Ly49H+ NK cells in blood at indicated days PI (left) and in indicated organs at day 28 PI (right). (D) Histograms of Ly49H expression on Ly49Hlo and Ly49Hhi NK cells from blood at day 7 PI. Data are representative of two independent experiments with 3–5 mice per experiment.

    Journal: Immunity

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells

    doi: 10.1016/j.immuni.2019.04.009

    Figure Lengend Snippet: (A-D) Experimental schematic (A). Equal numbers of splenic Ly49Hlo and Ly49Hhi NK cells, purified from congenically distinct WT mice, were co-transferred into Klra8−/− mice, and their responses tracked following MCMV infection of the recipients. (B) Flow plots gated on NK cells from blood at day 7 PI (left) and spleen at day 28 PI (middle). Quantification of percent Ly49Hlo and Ly49Hhi NK cells within total NK cells in blood at indicated days PI (right). (C) The percentage of Ly49Hlo and Ly49Hhi NK cells within transferred Ly49H+ NK cells in blood at indicated days PI (left) and in indicated organs at day 28 PI (right). (D) Histograms of Ly49H expression on Ly49Hlo and Ly49Hhi NK cells from blood at day 7 PI. Data are representative of two independent experiments with 3–5 mice per experiment.

    Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAF™ Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAF™ Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Techniques: Purification, Infection, Expressing

    KEY RESOURCES TABLE

    Journal: Immunity

    Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells

    doi: 10.1016/j.immuni.2019.04.009

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAF™ Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAF™ Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

    Techniques: Flow Cytometry, Purification, Recombinant, Staining, RNA Sequencing Assay, Expressing, Derivative Assay, Software, Imaging